Part:BBa_K5233022:Design

TurboPETase(OmpA), a designed enzyme that can be efficiently secreted into the extracellular space

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  COMPATIBLE WITH RFC[12]
• 21
  COMPATIBLE WITH RFC[21]
• 23
  COMPATIBLE WITH RFC[23]
• 25
  INCOMPATIBLE WITH RFC[25]

  Illegal AgeI site found at 220
  Illegal AgeI site found at 576
  Illegal AgeI site found at 621
  
• 1000
  COMPATIBLE WITH RFC[1000]

Design Notes

Since PET is extracellular, signal peptide is used here to reach the goal of secrete the enzyme to the extracellular environment for function. Besides, enhancer sequence is used to amplify the effect of signal peptide.Among all of the enzyme-siganl peptide combination we use ,TurboPETase(OmpA) has the greatest effect of degradation.

Source

The TurboPETase comes from an assay called "Computational redesign of a hydrolase for nearly complete PET depolymerization at industrially relevant high-solids loading". The ompA comes from outer membrane protein A. The enhancer comes from an assay called "Excretory expression of IsPETase in E. coli by an enhancer of signal peptides and enhanced PET hydrolysis".

References

Cui Y, Chen Y, Sun J, Zhu T, Pang H, Li C, Geng WC, Wu B. Computational redesign of a hydrolase for nearly complete PET depolymerization at industrially relevant high-solids loading. Nat Commun. 2024 Feb 15;15(1):1417. doi: 10.1038/s41467-024-45662-9. PMID: 38360963; PMCID: PMC10869840.